I seminari dipartimentali si svolgeranno secondo il seguente calendario, due volte al mese, dalle 13:00 alle 13:40 in aula seminari al II piano di largo Donegani 2 (ex Wild), a Novara.
Aggiungi gli eventi al tuo calendario cliccando qui https://goo.gl/jtddhG
1) Pharmacogenetics and genetics of migraine: our contribution to the field
03 ottobre 2018; Sarah Cargnin (UNIUPO - DSF)
Episodic migraine is a disabling neurovascular disease that affects around 10% of the worldwide adult population. Despite triptans are now considered as the gold-standard acute treatment for migraine, their use is limited by at least two main drawbacks: first, a consistent proportion of migraineurs do not benefit from these medications; second, it has been shown that the overuse of triptans per se is, paradoxically, one of the major causes of migraine chronification into Medication Overuse Headache (MOH). In the last years we conducted several studies aimed at identifying single neuclotide polymorphisms as genetic predictors of the clinical response to triptans or of the risk of developing MOH. We herein propose the use of a polygenic risk score approach as an adequate tool to unravel the genetic/pharmacogenetic basis of migraine and its chronification.
2) Role of the epigenetic modulators KDM6B and EZH2 in malignant mesothelioma
17 ottobre 2018; Giulia Pinton (UNIUPO – DSF)
Mesothelioma is a type of cancer that forms on the thin protective lining that cover the lungs and other internal organs, often associated with exposure to asbestos. For patients with relapsed or refractory mesothelioma, there are currently no approved treatment options.
Tazemetostat, a first-in-class EZH2 (Enhancer of Zeste homology 2) inhibitor, is currently being studied as a monotherapy in ongoing Phase 1 and 2 programs. EZH2 is a methyltransferase that trimethylates histone H3 lysine 27 (H3K27me3) on chromatin. This repressive mark is removed by the lysine demethylase KDM6B. We assessed the role of EZH2 and KDM6B in mesothelioma derived cells, cultured as monolayer or as spheroids.
3) From mass spectrometry to biochemistry: in vitro and in vivo applications
07 novembre 2018; Marcello Manfredi (UNIUPO – IRCAD)
Today, mass spectrometry methods are utilized in an increasing number of molecular and biology studies that aim for a better understanding of the biochemistry of living organisms. Biological processes, pathways and biomarkers are only some of the information that can be obtained through mass spectrometry. A selection of these studies will be presented: firstly, using a multi-omics approach we will show the specific behavior of serum proteins, metabolites and lipids during sport activity. The second case research is related to the use of proteomics to study the effect of a drug and its possible targets. The third case study will show the use of a multi-omics approach to analyze the content and the effect of platelet rich plasma.
4) MORPHEUS: an automated tool for unbiased and reproducible cell morphometry
21 novembre 2018; Federico Ruffinatti (UNIUPO – DSF)
Cell morphometry can be defined as the quantitative description of cell shape for the inference of important information about cell functionality and/or its correlation with certain biological process of interest, such as proliferation, differentiation, migration, motility, growth dynamics or adhesion to particular substrates. In recent years, a plethora of shape descriptors has been proposed to virtually capture every geometrical feature of a cell, or subcellular compartment. However, the simultaneous use of multiple descriptors rapidly raises the level of complexity, making the analysis multivariate and highly redundant. Moreover, the visual inspection of the whole dataset of images for manual cell selection, together with the lack of standardized protocols of analysis, is likely to introduce a heavy operator-dependent bias into the procedure, thus impairing its reproducibility. In order to solve most of these problems, we developed a new plug-in for ImageJ called MORPHEUS, that combines within a single pipeline the evaluation of the most relevant cell-shape descriptors, the extraction of nucleus features and an advanced analysis of orientation. Morpheus is a first proposal for the standardization of cell morphometric analysis in which the automation descending from the adaptative nature of the algorithm eliminates the user-bias factor and translates into a reproducibility-oriented approach to cell morphometry.
5) Relazioni struttura-attività del diterpene antitumorale EBC-46
05 dicembre 2018; Giovanni Battista Appendino (UNIUPO – DSF)
The diterpene ester tiglianol tigliate (EBC-46) occurs in high concentration in the unpalatable seeds of Fontainea picrosperma C.T. White (blushwood), a subcanopy euphorbiaceous tree endemic to a restricted (ca. 20 Km2) area of the Queensland rainforest. Tiglianol tigliate is remarkably efficacious in the treatment of localized tumors, and has successfully completed Phase III clinical development for veterinarian use and Phase I human clinical studies. Intra-tumor injection of EBC46 triggers a triad of responses (massive destruction of tumor vasculature, tumor hemorrhagic necrosis, stimulation of wound healing) sensitive to PKC pan-inhibitors, suggesting that modulation of PKC is part of its mechanism of action. The PKC isoform activation of EBC-6 is significantly different from the one of tetradecanoyl phorbol acetate (PMA) and ingenol mebutate, and studies aimed at clarifying the role of PKC in the mechanism of action of EBC-46 by systematically modifying the functional groups of the natural product will be presented.
6) Nucleotide Excision Repair and direct DNA damage reversal in Mycobacterium tuberculosis: a biochemical and structural perspective
19 dicembre 2018; Riccardo Miggiano (UNIUPO – DSF)
7) Real World Evidence: quanto è davvero reale?
09 gennaio 2019; Francesco Barone Adesi (UNIUPO – DSF)
8) A new possibility on acute myelogenous leukemia (AML): targeting myeloid differentiation using potent and innovative human dihydroorotate dehydrogenase (hDHODH) inhibitors
23 gennaio 2019; Marco Lolli (UNITO)
9) Lipotoxicity-mediated disruption of adult hypothalamic neural progenitor cells as a potential contributor in Metabolic Syndrome
06 febbraio 2019; Heather Bondi (UNIUPO – DSF)
10) Regulation of neuronal protein expression and function by astroglial calcineurin
20 febbraio 2019; Laura Tapella (UNIUPO – DSF)