Mercoledì 13 gennaio Seminari del DSF: "Isotopic labelings in biosynthetic studies – a first attempt to unravel tigliane diterpenes origin".
Il seminario è tenuto da Simone Gaeta (DSF-UNIUPO).
L'evento si svolgerà in modalità webinar dalle 13:00 alle 13:40, questo il link a cui collegarsi: meet.google.com/vur-yeoh-mci
The studies within natural products biosynthesis by the use of isotopes - stable or not- represented a milestone to unlock the chemical logic behind secondary metabolites production. Since the details of primary metabolism came into view, feeding experiments of isotopically enriched building blocks on plants tissue, microorganisms and fungi allowed to determine the connectivity of such fragments in complex natural scaffold. Despite the use of these techniques have become less central in the post-genome era due to bioinformatic predictions of ORF in established gene clusters, isotopomerguided studies are still a crucial card to play for chemists whenever the biosynthetic machinery is mostly unknown. A suitable case study is represented by tigliane diterpenoids (C20), a class of biomedical and technological relevant compounds naturally occurring in Euphorbiaceae and Thymelaceae families, whose biosynthesis is still considered a black-box in natural products chemistry. Driven by the elegant biogenetic proposal of prof. Jasmin Jakupovic (1949-2005), the synthesis of terpene precursors isotopologues (2H, 13C) and feeding experiments on the species Fontainea picrosperma C.T.White were performed as part of my PhD program. The analysis of the downstream archetypal molecule did not show a significant incorporation by LC-MS and NMR spectroscopy suggesting a metabolic-delay in the production of these noxious compounds in early stage of shootings life. The obtained results paved the way for new interdisciplinary experiments to solve the riddle of tigliane biosynthesis from a chemical to a biological point of view.